This study examined whether one PI-related enzyme, diacylglycerol kinase (DGK), which is regarded as a regulator of the DG signal through its metabolism, is expressed in mammalian retina. Enzymatic assay, Northern blot and RT-PCR analyses, and in situ hybridization histochemistry were performed to assess the expression profile of DGK isozymes and their cellular localization. In rat retina DGKε, DGKζ, and DGKι are the dominant species with distinct patterns of expression. At the cellular level, DGKε is the only one detected intensely in the photoreceptor layer, although DGKι and DGKζ are observed in bipolar and ganglion cell layers. These results suggest that each DGK isozyme plays a different role in the signal transduction in distinct cell types and that DGKε is a candidate involve...

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